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課程名稱 |
分子生物學及技術
Molecular Biology and Techniques |
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開課學期 |
102-1 |
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授課對象 |
醫學院 生理學研究所 |
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授課教師 |
林敬哲 |
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課號 |
md&ph5074 |
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課程識別碼 |
420 U4800 |
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班次 |
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學分 |
4 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期二8,9(15:30~17:20)星期四8,9(15:30~17:20) |
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上課地點 |
基醫301基醫301 |
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備註 |
本課程中文授課,使用英文教科書。研究所共同課程。與胡務亮、方偉宏、俞松良、邱浩傑、陳培哲、楊偉勛、林榮耀、張明富、呂紹俊、游偉絢、李明學、余明俊、張 鑫、董馨蓮、陳美如、鄧述諄、李財坤、葉秀慧、詹世鵬、楊宏志、李芳仁、阮麗蓉、徐立中、吳君泰、許秉寧、賈景
總人數上限：186人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1021mdph5074 |
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課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
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課程大綱
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課程概述 |
分子生物學及技術 |
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課程目標 |
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課程要求 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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第1週 |
9/10,9/12 |
(I) Introduction
(II) DNA structure and function
(1) DNA as store of information
(a) Outline of major experiments that led to the acceptance of DNA as the genetic material
(b) Structure of nucleic acid, base pairing, tautomeric shift, DNA-A,-B,-Z forms
(c) Denaturing and renaturing of DNA
(d) Supercoiled DNA and topoisomerase
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第2週 |
9/17,9/19 |
(2) DNA technology, genomic size, and genetic content |
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第3週 |
9/24,9/26 |
(3) Genome: organization and evolution
(4) DNA replication
(a) Eukaryotic system
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第4週 |
10/01,10/03 |
(b) Prokaryotic system
(5) Extrachromosome DNA, nucleosome, chromosome and chromatid
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第5週 |
10/08,10/10 |
(6) DNA damage and mutation
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第6週 |
10/15,10/17 |
(7) DNA repair and modification
(8) Chromosomal dynamics
Recombination , transposition, and rearrangement
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第7週 |
10/22,10/24 |
第一次期中考 (考試範圍9/10~10/17)
(III) Transcription, regulation and processing
(1) Prokaryotic transcription and eukaryotic pol II transcription
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第8週 |
10/29,10/31 |
(2) Transcription and epigenetic regulation
(3) Eukaryotic transcription and regulation (eukaryotic RNA pol I and III)
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第9週 |
11/05,11/07 |
(4) Post-transcriptional processing, ribozyme, and regulation
(5) Small RNA, micro RNA processing
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第10週 |
11/12,11/14 |
(IV) Translation
(1) Assembly line for protein synthesis
(2) Transfer RNA is the translated adaptor
(3) Ribosomes provide a translation factory
(4) Codons and their significance
(5) Translation in cell free system
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第11週 |
11/19,11/21 |
(6) Post-translational modification
(a) Structure
(b) Protein ubiquitination
(V) Signal transduction
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第12週 |
11/26,11/28 |
(VI) Genes in the development
(1) Immune diversity and immunotherapy
(2) Oncogenes
(3) Tumor suppressor genes
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第13週 |
12/03,12/05 |
(4) Gene regulation and development
第二次期中考(考試範圍 10/24~12/3)
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第14週 |
12/10,12/12 |
(VII) Recombinant DNA technology (分子生物學及技術包含以下課程)
(1) Cloning of DNA: transformation and screening of transformants
(2) Construction of cDNA and genomic libraries
(3) Colony and plaque hybridization, Southern blot, Northern blot, Western blot analysis
(4) Primer extension, S1 nuclease mapping and RNase protection assay
(5) Differential display
(6) Site-directed mutagenesis and polymerase chain reaction
(7) Application of restriction fragment length polymorphism and variable number tandem repeat for genetic disease analysis
(8) Genome-wide sequencing strategies
(9) Phage strategies: control of lytic and lysogenic pathway
(10) Phage display library
(11) A brief introduction to animal viruses
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第15週 |
12/17,12/19 |
(12) Expression of cloned gene in prokaryotic and eukaryotic cells
(13) Yeast genetics and its applications
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第16週 |
12/24,12/26 |
(14) Applications of bioinformatics and proteomics in biomedical research
(15) Current development and clinical application of genomics: personalized medicine
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第17週 |
12/31,1/02 |
(16) Transgenic techniques
(17) Gene therapy
(a) Viral vectors
(b) Clinical Application
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第18週 |
1/7 |
期末考 (考試範圍 12/10~01/02，修習分子生物學及技術者須參加考試) |
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